Application of pressure-strain loops in evaluating early left ventricular myocardial work in patients with metabolic syndrome / 中华超声影像学杂志

Objective:To evaluate early left ventricular myocardial work in patients with metabolic syndrome (MS) by pressure-strain loops (PSL).Methods:From September 2020 to April 2021, 70 MS patients in the First Hospital of Qinhuangdao Affiliated to Hebei Medical University without left ventricular remodeling were selected as MS group and 65 normal controls as control group, PSL was used to obtain the myocardial power parameters, including global work index (GWI), global work efficiency (GWE), global constructive work (GCW) and global wasted work (GWW). The changes of the parameters were compared between the two groups and their correlations with biochemical parameters were performed in MS group.Results:The left ventricular global longitudinal strain (GLS), GWI, GCW and GWE in MS group were lower than those in normal group, while GWW was higher than that in normal group ( P<0.05). Pearson correlation analysis showed that GWI was positively correlated with high-density lipoprotein cholesterol (HDL-C) ( r=0.194, P<0.05), and negatively correlated with diastolic blood pressure (DBP), waist circumference (WC), fasting blood glucose (Glu), triglyceride (TG) and GLS ( r=-0.257, -0.452, -0.239, -0.193, -0.758, all P<0.05). GWE was negatively correlated with SBP, DBP, WC, Glu, TG and GLS ( r=-0.360, -0.269, -0.326, -0.352, -0.265, -0.663, all P<0.01), and positively correlated with HDL-C ( r=0.201, P<0.05). GCW was negatively correlated with WC and GLS ( r=-0.299, -0.737; all P<0.001). GWW was positively correlated with SBP, DBP, WC, Glu, TG and GLS ( r=0.435, 0.308, 0.413, 0.547, 0.272, 0.400, all P<0.01), and negatively correlated with HDL-C ( r=-0.336, P<0.001). Conclusions:PSL can quantitatively evaluate the changes of early myocardial work in MS patients with good repeatability and has certain clinical value.

Diagnostic value of ultrasound in fetal posterior fossa anomalies at 11-13 + 6 gestational weeks / 中华超声影像学杂志

Objective:To explore the diagnostic value of ultrasound in posterior fossa anomalies (PFA) at 11-13 + 6 gestational weeks by measuring brainstem (BS), brainstem-to-occipital bone (BSOB) diameter and BS/BSOB ratio. Methods:A total of 209 normal fetuses (control group) were randomly selected from Beijing Obstetrics and Gynecology Hospital, Capital Medical University, between March 2018 and November 2021. Reference ranges for BS, BSOB diameter and BS/BSOB ratio were obtained on the mid-sagittal view of the fetal profile and the relationship of three parameters and crown-rump length (CRL) was investigated. The intra- and inter-observer reliabilities were determined by intraclass correlation coefficient (ICC) in 30 normal fetuses. Fourteen fetuses diagnosed with PFA in the same period including 10 cases of cystic posterior fossa malformations (cPFM) and 4 cases of open spine bifida (OSB) were retrospectively selected to compare BS, BSOB diameter and the BS/BSOB ratio with control group.Results:BS and BSOB diameters were successfully obtained in all control fetuses (100%), and the intra- and inter-observer reliabilities for BS and BSOB diameters were good (ICC=0.877, 0.846 and 0.939, 0.895). In the control group, BS and BSOB diameter linearly correlated with CRL ( r=0.867, 0.794; all P<0.001), while the BS/BSOB ratio was 0.75 (0.71, 0.79). There were significant differences of BSOB diameter and BS/BSOB ratio between control group and PFA group (all P<0.05). Except for one isolated vermian hypoplasia (VH), the BSOB diameters in 9 (90%) cases of cPFM were above the 95th percentile of the calculated normal range and were below the 5th percentile in 4(100%) cases of OSB.Except for one isolated VH, the BS/BSOB ratio in 9 (90%) cases of cPFM was below the 5th percentile of the calculated normal range. The BS/BSOB ratio in 4 (100%) cases of OSB was above the 95th percentile of the calculated normal range. Conclusions:The measurements of BS and BSOB diameter are feasible with good repeatability. Abnormal BSOB diameter and BS/BSOB ratio are suggestive for PFA. The posterior fossa of isolated VH can be normal in the first trimester.

Sonographic features of Joubert syndrome in first or second trimester: report of two fetuses / 中华围产医学杂志

We report two cases of Joubert syndrome initially tentatively diagnosed by prenatal ultrasound in the first or second trimester which were thereafter confirmed by whole exome sequencing (WES). Case 1 was one of the twins who presented with increased intracranial transparency (IT) and thinner brainstem at 12 +1 gestational weeks. Ultrasound at 18 +2 weeks found multiple intracranial malformations, "molar tooth sign (MTS)" at the midbrain-hindbrain junction level in the cerebral cross section, and bilateral ventriculomegaly. Enlarged and echogenic kidneys and oligohydramnios were also detected. In case 2, ultrasound image at 17 +5 weeks of gestation indicated multiple intra-and extra cranial and extracranial malformations, MTS in the midbrain-hindbrain junction plane, bilateral ventriculomegaly, unclear cavum septum pellucidum. Extracranial anomalies were bilateral multicystic enlarged kidneys, invisible bladder, and oligohydramnios. Both fetuses underwent amniocentesis, which showed normal karyotype and no copy number variation was detected. However, variation of the TMEM67 gene (c.312+5G>A at introns 2 and c.1175C>G at exon12) was detected in both fetuses by WES, supporting the diagnosis of Joubert syndrome. Selective reduction and termination of pregnancy were performed on case 1 and case 2 at 18 +5 and 19 weeks of gestation, respectively.

Removal of nonimpacted third molars alters the periodontal condition of their neighbors clinically, immunologically, and microbiologically / 国际口腔科学杂志·英文版

Considering the adverse effects of nonimpacted third molars (N-M3s) on the periodontal health of adjacent second molars (M2s), the removal of N-M3s may be beneficial to the periodontal health of their neighbors. This study aimed to investigate the clinical, immunological, and microbiological changes of the periodontal condition around M2s following removal of neighboring N-M3s across a 6-month period. Subjects with at least one quadrant containing an intact first molar (M1), M2, and N-M3 were screened and those who met the inclusion criteria and decided to receive N-M3 extraction were recruited in the following investigation. M2 periodontal condition was interrogated before M3 extraction (baseline) and at 3 and 6 months postoperatively. Improvements in clinical periodontal indexes of M2s in response to their adjacent N-M3 removal, along with changes in inflammatory biomarkers among gingival crevicular fluid (GCF) and the composition of subgingival plaque collected from the distal sites of the M2s of the targeted quadrant were parallelly analyzed. Complete data of 26 tooth extraction patients across the follow-up period were successfully obtained and subsequently applied for statistical analysis. Compared to the baseline, the periodontal condition of M2s was significantly changed 6 months after N-M3 removal; specifically, the probing depth of M2s significantly reduced (P < 0.001), the matrix metalloproteinase (MMP)-8 concentration involved in GCF significantly decreased (P = 0.025), and the abundance of the pathogenic genera unidentified Prevotellaceae and Streptococcus significantly decreased (P < 0.001 and P = 0.009, respectively). We concluded that N-M3 removal was associated with superior clinical indexes, decreased GCF inflammatory biomarkers, and reduced pathogenic microbiome distribution within the subgingival plaque. Although the retention or removal of N-M3s continues to be controversial, our findings provide additional evidence that medical decisions should be made as early as possible or at least before the neighboring teeth are irretrievably damaged.

Quantitative evaluation of normal fetal posterior fossa anatomy using transabdominal three-dimensional ultrasound / 中华超声影像学杂志

Objective:To analyze the consistency and repeatability for quantitative evaluation of normal fetal posterior fossa anatomy using transabdominal three-dimensional ultrasound, and assess the clinical value.Methods:The midsagittal planes of fetal brain from 127 normal singleton fetuses with 22-34 weeks of gestation were obtained using transabdominal three-dimensional ultrasound between May, 2020 and May, 2021. The quantitative three-dimensional indicators were measured by two observers, then were measured twice by one of the two observers. The double blind was performed in course of measuring procedures. The intraobserver and interobserver agreements were evaluated. The relationships between three-dimensional indicators and gestational age were analyzed.Results:The quantitative three-dimensional indicators expressing the size of vermis, including area, perimeter, suprainferior diameter and anteroposterior diameter, had positive correlations with advanced gestational age ( r=0.934, 0.936, 0.920, 0.879; all P<0.001). The intra- and interobserver reproducibilities of measurements were very good with all intraclass correlation coefficients >0.80 (all P<0.001). Brainstem-vermis (BV) angle and Brainstem-tentorium (BT) angle had negative and positive correlations with advanced gestational age respectively ( r=-0.317, 0.366; both P<0.001). The intra-and interobserver reproducibilities of measurements were moderate or poor. Conclusions:The quantitative three-dimensional indicators describing the size of vermis are worthy of clinical application for evaluation of fetal posterior fossa owing to the excellent reproducibility and simple and feasible method of measurement. BV angle and BT angle are not applicable for junior physicians due to the poor reproducibility of measurement.

Trends of loss of peripheral muscle thickness on ultrasonography among ICU patients / 中国实用护理杂志

Objective:To describe the trends of loss of muscle fiber thickness of the arm and calf.Methods:From April 2019 to July 2019, 58 patients admitted to ICU of Qinghai Provincial People's Hospital were enrolled. During ICU stay, the muscle fiber thickness in bilateral upper arm (biceps brachii) and bilateral calf (gastrocephus muscle) were measured by bedside color ultrasound every day.Results:The muscle fiber thickness of biceps and gastrocnemius on the left and right were (1.52 ± 0.37), (1.50 ± 0.34), (1.53±0.39), (1.51 ± 0.37) mm, respectively. The thickness of muscle fibers were (1.45 ± 0.35), (1.46 ± 0.37), (1.44±0.33), (1.41 ± 0.32) mm, which were significantly decreased in 48 h admitted to ICU ( t values were 2.106-4.711, P<0.05 or 0.01); and (1.43 ± 0.36), (1.44 ± 0.36), (1.44±0.32), (1.39 ± 0.32) mm in 72 h, which were significantly decreased than 24 h admitted to ICU ( t values were2.029-4.504, P<0.05 or 0.01). During 1 week admission to ICU , the muscle fiber thickness showed a continuous trend of decline, and the muscle fiber thickness of the left biceps, right biceps, left gastrocnemius and right gastrocnemius decreased by 8.38% (0.13/1.55), 10.19% (0.16/1.57), 9.87% (0.15/1.52), 11.11% (0.17/1.53), respectively compared with the ICU admission. The muscle fiber thickness of the left biceps, right biceps, left gastrocnemius and right gastrocnemius decreased by 9.87% (0.15/1.52), 9.33% (0.14/1.50), 9.15% (0.14/1.53), 11.26% (0.17/1.51), respectively. Conclusion:Muscle attenuation can be observed within 48h after ICU entry, and it tends to decrease with the extension of the length of hospital stay.

Inhibition of MicroRNA-15a/16 Expression Alleviates Neuropathic Pain Development through Upregulation of G Protein-Coupled Receptor Kinase 2

There is accumulating evidence that microRNAs are emerging as pivotal regulators in the development and progression of neuropathic pain. MicroRNA-15a/16 (miR-15a/16) have been reported to play an important role in various diseases and inflammation response processes. However, whether miR-15a/16 participates in the regulation of neuroinflammation and neuropathic pain development remains unknown. In this study, we established a mouse model of neuropathic pain by chronic constriction injury (CCI) of the sciatic nerves. Our results showed that both miR-15a and miR-16 expression was significantly upregulated in the spinal cord of CCI rats. Downregulation of the expression of miR-15a and miR-16 by intrathecal injection of a specific inhibitor significantly attenuated the mechanical allodynia and thermal hyperalgesia of CCI rats. Furthermore, inhibition of miR-15a and miR-16 downregulated the expression of interleukin-1β and tumor-necrosis factor-α in the spinal cord of CCI rats. Bioinformatic analysis predicted that G protein-coupled receptor kinase 2 (GRK2), an important regulator in neuropathic pain and inflammation, was a potential target gene of miR-15a and miR-16. Inhibition of miR-15a and miR-16 markedly increased the expression of GRK2 while downregulating the activation of p38 mitogen-activated protein kinase and NF-κB in CCI rats. Notably, the silencing of GRK2 significantly reversed the inhibitory effects of miR-15a/16 inhibition in neuropathic pain. In conclusion, our results suggest that inhibition of miR-15a/16 expression alleviates neuropathic pain development by targeting GRK2. These findings provide novel insights into the molecular pathogenesis of neuropathic pain and suggest potential therapeutic targets for preventing neuropathic pain development.

Speckle tracking imaging in evaluation of early myocardial injury of sepsis rats and effect of naringin pretreatment / 中国医学影像技术

Objective: To investigate the value of speckle tracking imaging (STI) technique in evaluating lipopolysaccharide (LPS) induced early myocardial injury in sepsis rats and naringin (Nar) pretreatment in reversing myocardial injury. Methods: Thirty-six SD rats were randomly divided into LPS+Nar50 group (n=9), LPS+Nar100 group (n=9), LPS group (n=10) and control group (n=8). Rats in LPS+Nar50 group and LPS+Nar100 group were given Nar suspension by gavage with 50, 100 mg/kg, respectively, while in other two groups were given the same amount of normal saline. Continuous gavage was performed for 7 days, then rats in LPS +Nar50 group, LPS+Nar100 group and LPS group were intraperitoneally injected with 5 mg/kg LPS 1 hour after the last gavage, while in control group were given the same amount of normal saline. Six hours after LPS injection, all rats were tested for left ventricular ejection fraction (LVEF) and Tei index, peak circumferential strain (SC) at each segment of the left ventricle (LV), LV global subendocardial, middle and subepicardial SC (respectively for GSCendo, GSCmid, GSCepi), systolic peak rate of SC (SrC S), peak early diastolic SC rate (SrC E), late diastolic peak rate of SC (SrC A). Serum levels of creatine kinase (CK) and lactate dehydrogenase (LDH) were detected, and pathological changes of myocardial tissue were observed by HE. Results: The peaks of SC at each segment of LV in LPS group were lower than those in control group and LPS+Nar100 group (all P<0.05). In LPS+Nar50 group, SC peaks of anterior septum, anterior wall, inferior wall and posterior septum were higher than those in LPS group but lower than those in LPS+Nar100 group: SC peak of lateral wall in LPS+Nar50 group was lower than that in LPS+Nar100 group (all P<0.05). LVEF, GSCendo, GSCmid, SrC S, SrC E and SrC A in LPS group were significantly lower than the other three groups, and Tei index, CK and LDH were all higher than in the other three groups (all P<0.05). LVEF, GSCmid, SrC S, SrC E and SrC A in LPS+Nar50 group were lower than those in LPS+Nar100 group, while Tei index, CK and LDH were all higher than those in LPS+Nar100 group (all P<0.05). Pathological results showed that some of the cells in LPS group were pyknotic and hyperemic, and the inflammatory cells increased. The above pathological changes significantly reduced in LPS+Nar50 group and LPS+Nar100 group compared with LPS group, and the reduction was more significant in LPS+Nar100 group. Conclusion: STI can be used to evaluate early myocardial injury and Nar pretreatment on reducing myocardial injury in sepsis rats.

Effect of the application of nursing quality sensitive indicators in burn patients / 中国实用护理杂志

Objective To investigate the effect of the application of nursing quality sensitive indicators on the nursing care of burn patients and their satisfaction with nursing care. Methods A total of 90 cases of burn patients admitted from January 2015 to December 2016 were selected as study subjects. They were divided into pre- implementation group (January 2015- December 2015) and implementation group (January 2016-December 2016) for 45 cases each based on sensitive indicators of care quality. The traditional quality management control method was adopted before implementation. The quality-sensitive indicators of nursing quality were used for quality management after the implementation. The quality monitoring indicators, nursing adverse events, and nursing satisfaction were compared before and after implementation. Results After the implementation, the implementation rate of graded nursing, the accuracy of patient identification, the qualified rate of qualified nurses, the passing rate of risk assessment, and the passing rate of high-risk drugs were 97.78%(44/45), 93.33%(42/45), 95.56%(43/45), 97.78% (44/45), and 97.78% (44/45), which were significantly higher than those before implementation 84.44%(38/45)、77.78%(35/45), 82.22%(37/45), 82.22%(37/45), and 84.44%(38/45), respectively, and the difference was statistically significant (χ2= 4.050-, 6.049, P<0.05). After the implementation, drug errors and other adverse events were used. The incidence rate of 0.22%(1/450)was significantly lower than the pre-implementation rate of 1.78%(8/450), and the difference was statistically significant (χ2=5.499, P<0.05). After implementation, patient satisfaction with service attitude, basic nursing, professional skills, and comprehensive nursing service quality was 95.56%(43/45), 97.78%(44/45), 95.56%(43/45), 97.78%(44/45), and 95.56%(43/45), which were significantly higher than those before implementation 88.89%(40/45), 84.44%(38/45), 82.22%(37/45), 80.00%(36/45)and 82.22% (37/45), and the difference was statistically significant (χ2= 4.050-7.200, P <0.05). Conclusion The application of sensitive indicators of nursing quality can help improve the care quality of burn patients, reduce the occurrence of adverse care events, and enhance patient satisfaction.

Spectral Doppler Ultrasound in Evaluating Haemodynamic Change of Fetus with Nervous System Abnormalities / 中国医学影像学杂志

Purpose To assess the haemodynamic change of fetus with nervous system abnormalities by using spectral Doppler ultrasound.Materials and Methods Twenty-two fetuses with nervous system abnormalities diagnosed by ultrasound examination in Beijing Obstetrics and Gynecology Hospital,Capital Medical University,September,2015 to May,2016 were chosen.Fetus gestational week was 20-24 weeks.All Fetuses with abnormalities in nervous system were confirmed by autopsy and image examination after birth.Meanwhile,50 cases of normal fetuses with same gestational week were chosen as control group.Assessment indexes of spectral Doppler ultrasound between the two groups were compared,including myocardial performance index (MPI) of left ventricle (automatic survey),middle cerebral artery (MCA),umbilical artery (UA) and heart rate.Results MPI in control group and in simple nervous system abnormal group were compared,the difference was statistical significant (0.44±0.05 vs 0.48±0.08,P＜0.05).MCA resistance index in the two groups showed statistically significant difference (0.80±0.06 vs 0.88±0.14,P＜0.05).The difference of other indexes showed no statistical significant (P＞0.05).Conclusion MPI automatic survey is reliable and convenient for assessing cardiac function of fetus.There is a certain change of haemodynamic for fetus with abnormal nervous system and normal ones,which is represented by decline of fetus cardiac function and increase of MCA resistance.Spectral Doppler ultrasound can provide more information and evidences for intrauterine monitoring of fetus with abnormal nervous system.

Mutation analysis of autosomal and X chromosomal STR in gynecologic and breast cancer / 中国法医学杂志

Objective To explore the mutation types and disciplines of STR commonly used in forensic in gynecologic and breast cancerand investigate the application of microdissection in forensic practice involving tumor tissue. Methods DNA of tumor tissues, adjacent normal tissues and peripheral blood from 62 patients with breast cancer, 62 patients with gynecologic cancer and 10 patients with benign gynecologic tumor were amplified by PowerPlex 21 System kit and Argus X-12 kit. Capillary electrophoresis of PCR products was carried out on an ABI 3130 Genetic Analyzer to obtain genotypes. Some tumor tissues with STR variation were microdissected. Results The genotype of peripheral blood in cancer patient was consistent with that of corresponding normal tissue. 4 types of STR variations were found in 46.77% gynecologic cancer tissues, compared with that in benign tumor tissues and breast cancer, the difference of STR variation was significant(P<0.01,P=0.009). The genotype of stromal cells separated by microdissection was consistent with that of corresponding adjacent normal tissue. Conclusion The STR loci detected in the study with poor stability are not suitable for forensic cases involving gynecologic cancer tissues. The genotype of stromal cells separated accurately from tumor tissues by microdissection could represent the normal DNA genotype of the individual with cancer. Microdissection is an effective solution in forensic cases with tumor tissues.

Comparison of detection of SNP and STR in formalin-fixed tissue / 中国法医学杂志

Objective The purpose of this study was to detect the degradation degree of long-term formalin fixed tissue and to compare the detection rate of STR with SNP. Methods DNA was extracted from 24 formalin-fixed tissues stored at -20 ℃ for five years, and the concentration and degradation index of DNA was quantified with Quantifiler? Trio DNA Kit. A 55-SNP multiplex SNaPshot assay and PowerPlex? 21 system were used to amplify SNP and STR loci, respectively. Results The results showed that the degradation indexes of 24 specimens were ranged from 1~8. The SNP genotypes of the 24 specimens were completely consistent with the non-degraded DNA from the same individuals and the successful genotyping rate was 100%. However, 33 allele dropouts were observed with STR genotyping in 8 samples, of which the degradation index was higher than 2.6, and the fragment size of the 75.8% allele was longer than 300bp. The likelihood ratio based on 16 typable STR loci in the sample was close to that onthe basis of 54 SNPs. However, likelihood ratio based on more than 17 STR loci was over that accord to 54 SNPs. There was a negative correlation between the fragment size of STR and the allele detection rate, and a negative correlation also observed between the degradation index of samples and the allele detection rate except for two samples with mild degradation. Conclusion This study validated that the long-term formalin-fixed tissues were susceptible to degradation, and the SNP was more suitable for detecting these tissues than STR typing system. However more SNP loci are needed to test in order to increase the discrimination power.

Value of cervical stiffness measured by trans-vaginal ultrasound and real-time sono-elastography for predicting preterm labor in pregnant women / 中华医学超声杂志（电子版）

Objective To assess the application value of trans-vaginal ultrasound and real-time sono-elastography in assessing the risk of preterm birth in pregnant women. Methods A total of 108 pregnant women who received prenatal examination at Beijing Obstetrics and Gynecology Hospital from January to November 2017 were included. Based on the presence of threatened premature or preterm labor or not, they were divided into a study group (n=29, with threatened premature or preterm labor) and a control group (n=79, with no threatened premature or preterm labor). Cervical length (CL) was measured by trans-vaginal ultrasound, and the following three cervical elasticity indexes were obtained by sono-elastography: the entire cervical strain rate (CS), the internal cervical os strain rate (IS), and external cervical os strain rate (ES). Independent samples t-test was used to analyze the difference in the above indexes between the two groups. Receiver operating characteristic (ROC) curve analysis was performed to assess the value of the above indexes in predicting the risk of preterm birth.Results CL was significantly lower[(2.94±0.66)cm vs(3.91±0.71)cm]and IS was significantly higher [(0.53±0.28)% vs (0.47±0.20)%] in the study group than in the control group (t=6.436, 3.406,both P<0.05).CS and ES in the study group were higher than those in the control group[(0.40±0.30)% vs (0.24±0.19)%,(0.74±0.39)% vs(0.73±0.37)%],but the differences were not statistically significant(t=1.365, 0.207, both P>0.05). ROC curve analysis indicated that CL, CS, IS, and ES performed differently in assessing the risk of preterm birth in pregnant women [area under the ROC curve (AUC): 0.156, 0.558, 0.689, 0.499]. The AUCs of CS and IS were greater than 0.50, and the AUC of IS was larger than those of other indexes. Using 0.30% as a cut-off for IS, its sensitivity, specificity, accuracy, positive predictive value, and negative predictive value were 66.7%, 79.3%, 71.1%, 55.4%, and 80.2%, respectively. Conclusions Cervical tissue elasticity indexes obtained by sono-elastography can be used to assess pregnant women at different gestational ages and predict the risk of preterm birth feasibly. IS is more useful than other indexes in predicting early or asymptomatic preterm birth.

The correlation between three-dimensional histogram vascular indexes of ovarian tumors and microvessel density / 中华医学超声杂志（电子版）

Objective To evaluate the correlation between three-dimensional histogram vascular indexes of ovarian tumors and microvessel density (MVD).Methods Fifty-seven patients with ovarian cystic-solid or solid masses from Beijing Obstetrics and Gynecology Hospital,Capital Medical University were evaluated by three-dimensional (3D) power ultrasound within one week before surgery from January 2008 to December 2009.We captured 3D power imaging in the interested and solid areas of tumors,and used three-dimensional histogram from virtual organ computer-aid analysis (VOCAL) to calculate vascularization index (Ⅵ),flow index (FI) and vascularization-flow index (VFI).After operation the specimens underwent immunohistochemistry staining and MVD and vascular endothelial growth factor (VEGF) were calculated.Two independent-samples t tests were used to compare MVD between ovarian benign and malignant tumors.Rank sum test was used to compare VEGF.Spearman was used to analyze the relationship between 3D PD vascular indices and MVD.Results Sixty-two ovarian masses in 57 patients were confirmed by pathology (5 cases were bilateral tumors),including 22 benign and forty malignant messes (including eight borderline tumors).Fifty-four tumors underwent immunohistochemistry staining.MVD and VEGF were calculated.Both of MVD and VEGF in ovarian malignant tumors were obviously higher than those in benign ones (t=-5.255,Z=-3.868,both P ＜ 0.01).Both of Ⅵ and VFI were positive correlative to MVD (r=0.295,0.280,both P ＜ 0.05).Conclusions There was a positive correlation between vascular indexes and microvessel density.The vascular indexes could reflect the density of angiogenesis in ovarian tumors before surgery,and they could provide valuable information for early-stage diagnosis of ovarian cancer.

The preoperative evaluation value of three-dimensional ultrasound imaging for ovarian masses / 中华医学超声杂志（电子版）

Objectives To explore the clinical value of preoperative three-dimensional ultrasound imaging for ovarian tumors for reflecting the anatomy postoperatively.Methods Forty-seven patients with ovarian cystic-solid or solid masses from Beijing Obstetrics and Gynecology Hospital,Capital Medical University were evaluated by two-dimensional (2D) and three-dimensional (3D) ultrasound examination within one week before surgery from January 2008 to December 2009.Every ovarian mass was examined by three-dimensional ultrasound and tomographic ultrasound imaging (TUI) and the results was compared with the specimen postoperatively.Results Forty-seven ovarian masses were confirmed by pathology,including 23 benign masses and 24 malignant masses (six borderline tumors).Compared with the postoperative specimens,the accuracy of preoperative 3D ultrasound imaging of ovarian tumors for the anatomical structures was 89.4％ (42/47).The misdiagnosis causes in 5 cases included failure to detect tiny papillae on the Sturface and small cysts in the mass,failure to display the compressed structures accurately,wrongly believe the teeth on the tumor wall as papillae and wrongly believe two adjacent ovarian tumors as one unilateral mass.Conclusions Compared with postoperative specimens,the anatomy of ovarian tumors could be reflected accurately by 3D ultrasound imaging preoperatively.It could be helpful for differential diagnosis between benign and malignant ovarian tumors and the decision of surgical plan.

Risk factors of polyp recurrence after colorectal polypectomy / 中华消化内镜杂志

Objective To explore the relationship between polyp features at first-time colonoscopy and the recurrence, and to analyze the risk factors of recurrence at different time points of follow-up. Methods The data of 614 patients undergoing colorectal polypectomy between May 2008 and May 2016 were retrospectively analyzed. Patients were classified into 3 groups according to the characteristics and polyp features at first-time colonoscopy. The risk factors influencing polyp recurrence at different time points during follow up were analyzed. Results Univariate analysis showed that age ≥70 years, polyp′s diameter ≥0.5 cm,the number of polyps >2 and distribution throughout colon were risk factors for recurrence. In multivariate models,the number of polyps at baseline was the only significant predictor for recurrence(OR=2.36,95%CI:1.06-5.25). All of 614 patients underwent 6-87 months surveillance colonoscopy. The total recurrence rate was 58.6%(360/614). During four different surveillance intervals including 6-24 months,>24-36 months, >36-48 months, and >48-87 months,the cumulative recurrence rate of high-risk group was 60.1%,65.7%,80.7%,and 83.8%,respectively,whereas,that of low-risk group was 22.7%,40.0%, 53.8%,and 65.4%, respectively. There was a significant difference between the two groups(P=0.00). Conclusion The number of initial colorectal polyps is useful for predicting the risk of polyp recurrence,and the rate of polyp recurrence during surveillance increases with the passage of time. The cumulative recurrence rate of high-risk group after polypectomy is significantly higher than that of low-risk group.

Evaluation on effect of rosuvastatin in treatment of lower extremity atherosclerotic disease in elderly / 吉林大学学报(医学版)

Objective To observe the clinical effect of rosuvastatin in the treatment of lower extremity atherosclerotic disease in the elderly,and to provide clinical basis of the application of statins in lower extremity atherosclerotic disease in the elderly.Methods 90 patients diagnosed as lower extremity atherosclerotic disease (aged 60 years or over)were randomly divided into simvastatin group and rosuvastatin group (n=45),and they were treated with simvastatin and rosuvastatin,respectively,based on the regular treatment.The total cholesterol (TC),low density lipoprotein-cholesterol (LDL-C), and high sensitivity C-reactive protein (hs-CRP)of the patients in two groups were detected at the begining of treatment and the ending of 32 weeks;at the same time,the lower limb artery intima thickness, strength of hardened, plaque, and artery stenosis degree scores were compared.Results 32 weeks after treatment,the levels of TC,LDL-C and hs-CRP of the patients in two groups were decreased statistically compared with before treatment (P <0.05);the levels of TC,LDC-C,and hs-CRP in rosuvastatin group were lower than those in simvastatin group (P < 0.05 ). 32 weeks after treatment in two groups,compared with before treatment,the lining thickness and plaque,stenosis scores were decreased (P <0.05);the lining thickness,plaque and stenosis scores in rosuvastatin group were lower than those in simvastatin group (P <0.05);the gardening strength grades had no statistical difference between before and after treatment. Conclusion Statins is lipid-lowering and resistant to lower limb arteriosclerosis,and rosuvastatin is better than simvastatin.

Calycosin-7-O-β-d-glucopyranoside stimulates osteoblast differentiation through regulating the BMP/WNT signaling pathways

The isoflavone calycosin-7-O-β-d-glucopyranoside (CG) is a principal constituent of Astragalus membranaceus (AR) and has been reported to inhibit osteoclast development in vitro and bone loss in vivo. The aim of this study was to investigate the osteogenic effects of CG and its underlying mechanism in ST2 cells. The results show that exposure of cells to CG in osteogenic differentiation medium increases ALP activity, osteocalcin (Ocal) mRNA expression and the osteoblastic mineralization process. Mechanistically, CG treatment increased the expression of bone morphogenetic protein 2 (BMP-2), p-Smad 1/5/8, β-catenin and Runx2, all of which are regulators of the BMP- or wingless-type MMTV integration site family (WNT)/β-catenin-signaling pathways. Moreover, the osteogenic effects of CG were inhibited by Noggin and DKK-1 which are classical inhibitors of the BMP and WNT/β-catenin-signaling pathways, respectively. Taken together, the results indicate that CG promotes the osteoblastic differentiation of ST2 cells through regulating the BMP/WNT signaling pathways. On this basis, CG may be a useful lead compound for improving the treatment of bone-decreasing diseases and enhancing bone regeneration.

Value of nuchal translucency thickening in the fetal chromosome abnormality screening / 中华妇产科杂志

Objective To investigate the value of nuchal translucency(NT) thickening in the fetal chromosome abnormality screening.Methods The 14 881 pregnant women received NT measurement in 11-13 +6 weeks at Beijing Obstetrics and Gynecology Hospital from January 2010 to August 2012.The 118 fetuses whose NT≥2.5 mm were recruited.One hundred and eight (91.5％,108/118)of them accepted invasive procedure and karyotype analysis.Results (1) Chromosome karyotype analysis:113 singleton pregnancies (95.8％) and 5 twin pregnancies (4.2％) whose NT thickened from 2.5 mm to 11.0 mm were advised karyotype analysis.The 108 pregnant women accepted karyotype analysis.Among them,88 had normal chromosome karyotype,and 20 had chromosome abnormalities.The detection rate was 18.5 ％ (20/108).(2) The sensitivity,specificity,false positive rate,false negative rate,total consistent rate,positive predictive value and negative predictive value of prenatal screening of chromosome abnormalities for NT ≥ 2.5 mm were 44％,99％,1％,56％,99％,19％ and 100％,respectively.(3) Among the 88 fetuses who had normal karyotype,72 (82％) had isolated thickened NT,while 16 (18％) had fetal structural malformation or intrauterine demise.(4) Among the 10 pregnant women who did not accept fetal karyotype analysis,8 terminated pregnancy because of fetal structural malformation,and the other 2 fetuses died in uterus.(5)All of the 5 twin pregnancies were dichorionic twins,and one of the twins had thickened NT.Among the 5 twin pregnancies,one fetus was trisomy 21 and others had normal karyotype.3 twin pregnancies who had normal chromosomes gave live birth.And the other had a gastroschisis and exstrophy deformity fetuse.This fetuse died in uterus and remaining fetuse had full-term live birth.(6) 35 (29.7％) pregnant women received second trimester Down's syndrome serum screening.One was high risk and was proved a trisomy 21 pregnancy.Thirty-four were low risk and had normal chromosome karyotype.Conclusion NT thickening may indicate fetal chromosome abnormalities or other structural malformations.It is a useful prenatal screening indicator.